Coding
off elemen

Part:BBa_K4841005:Design

Designed by: Dai Yuchen   Group: iGEM23_TSBC-SZ   (2023-08-12)


esal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 98
    Illegal NgoMIV site found at 353
    Illegal AgeI site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Amplified from pAC-EsaRI plasmid and was appended to the amplicons containing promoters and RBS variants using overlap extension PCR.

References

1. Shong, J. & Collins, C.H. Engineering the esaR promoter for tunable quorum sensing- dependent gene expression. ACS Synth. Biol. 2, 568–575 (2013).
2. Gupta, A., Reizman, I., Reisch, C. et al. Dynamic regulation of metabolic flux in engineered bacteria using a pathway-independent quorum-sensing circuit. Nat Biotechnol 35, 273–279 (2017). https://doi.org/10.1038/nbt.3796